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Description
Rat HSP60 (Heat Shock Protein 60) ELISA KitThe Rat (HSP60) Heat Shock Protein 60 ELISA Kit measures Heat Shock Protein 60 in Rat samples. The plate has been pre coated with Rat HSPD1 antibody. HSPD1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HSPD1 Antibody is added and binds to HSPD1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HSPD1 antibody. After incubation unbound Streptavidin HRP is washed away
The Rat (HSP60) Heat Shock Protein 60 ELISA Kit measures Heat Shock Protein 60 in Rat samples. The plate has been pre-coated with Rat HSPD1 antibody. HSPD1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HSPD1 Antibody is added and binds to HSPD1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HSPD1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HSPD1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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